Equipment of Aerobic Plate Count

Item No.: YTEST-4-4158

This article introduces the procedure and equipment for aerobic plate count in food. The equipment can meet the needs of aerobic plate counting.

Product parameters
Product Name:	Equipment of Aerobic Plate Count
Model Number:	TEST-2
Brand:	FOOD EYES
Minimum Order Quantity:	1 single equipment or instrument for aerobic plate count.
Price:	The price is determined according to the parameters and quantity of the order.
Supply Ability:	15 sets (complete sets) of equipment of aerobic plate count per month.
Place of Origin:	China

INQUIRY

Description

This article introduces the procedure and equipment for aerobic plate count in food. The equipment can meet the needs of aerobic plate counting.

Bacteria are so small that they are hard to see with the naked eye. But after they are cultured, they grow from a single bacterium to a colony, and these colonies are easy to see and count.

1. Procedure of Aerobic Plate Count Method

2. Calculation of Aerobic Plate Count
2.1 Use a magnifying glass or an automatic colony counter to observe and count the colonies on the plate culture medium.
2.2 Expressed as "colony-forming units, CFU".
2.3 Select the plate culture medium with colonies between 30 CFU to 300 CFU without abnormal colonies to count the number of colonies. The plates below 30 CFU recorded in true colony counts, and the plates above 300 CFU recorded as "too numerous to count (TNTC)". The number of colonies for each degree of dilution shall be the average of two plates.
2.4 Calculation formula: N = ∑c / [(1 × n1) + (0.1 × n2)] × d
In the formula:
N ---- the number of colonies per gram or milliliter of the sample;
∑c ---- the sum of the number of colonies on the plate that can be counted;
n1 ---- the number of plate that can be counted in the first degree of dilution ;
n2 ---- the number of plate that can be counted in the second degree of dilution ;
d ---- The lowest dilution of the count.

3. Cautions for Aerobic Plate Count Method
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3.1 All tools that contact the contents of the sample should be sterilized by Horizontal Laboratory Autoclave Sterilizers or Vertical Laboratory Autoclave Sterilizers.
3.2 The operation of the sample opening or after opening the lid shall be carried out in an air-cleaned environment, that is aseptic operation. The aseptic operation can be performed in a sterile laboratory (clean room) or clean benches.
3.3 If the contents are a mixture of solid and liquid, put them in Sterile Sample Blender Bags, seal the bags, pat with stomacher blenders, break the solids of the contents, and then inspect.
3.4 If the contents are multiple liquids and you want to stir evenly, use a Magnetic Stirrer Mixer with Timer and HotPlate or a 4 Heads Magnetic Stirrer Mixer with HotPlate or Multifunctional Orbital Shaker Incubators for Laboratory, to ensure that there are no contaminated by microorganisms when are stirring.
3.5 A blank test is required (sample test of no microorganisms). When there is a microbe in the blank test, it indicates that there is a problem with the test operation and the result is not credible.

4. Equipment and Instruments for Aerobic Plate Count Method
4.1 Automatic colony counter: Photograph, analyze and count colonies on plate culture media.
4.2 PSB Biochemical Incubators for Laboratory: The sample is subjected to microbial culture. Temperature accuracy is ±1℃. There are cooling and heating functions.
4.3 Benchtop Water Baths for Laboratory: Some samples are better for microbial culture in a constant temperature water bath. Temperature accuracy is ±1℃. It can also be used in Water Bath Orbital Shakers for Laboratory or Laboratory Refrigeration and Heating Circulating Water Bath Chiller or Water Bath of Three Independent Temperatures for Laboratory.
4.4 Laboratory Analytical Balances or Precision Balances: Accurately weigh the sample.
4.5 Benchtop pH Meter Tester: Measure the pH of the sample or culture.
4.6 Sterilized SCO-1 Sanitary Cans Opener: It does not destroy the double seam when open the lid of the can.
4.7 JV1 Clean Benches or Class 100 sterile laboratory (clean room): The operation is carried out in a clean environment, that is aseptic operation.
4.8 Pipettors or Micropipettes Autoclavable or Pasteur Pipettes and Disposable Droppers or glass pipette: Remove the liquid from the sample.
4.9 PYM-01 Glass Petri Dishes for Cell Culture or Glass Test Tubes with Rim Roll or Ordinary Glass Erlenmeyers and Conical Flasks: For the production of media.
4.10 Metal inoculation Loops and handles: Can pick, transfer and inoculate microorganisms during inoculation. The metal inoculation loop can be reused after sterilization.
4.11 Infrared Sterilizers for Inoculation Loops and Needles: Uses electric heating to sterilize the inoculation loop and needle. The infrared inoculation sterilizer has no open flame and is not afraid of wind, is practical testing equipment of microbiology.
4.12 If you need other laboratory equipment, please browse the “Laboratory Equipment and Instruments”, “Chemistry Laboratory Glassware”, “Food Safety Testing and Inspection Equipment” or “Can Testing Equipment Instruments”.

5. Note
• To learn more about the principles of aerobic plate count, you can read " Method of Aerobic Plate Count".
• Most countries have provisions for aerobic plate count method, but the regulations for each country may vary. This article mainly refers to the relevant aerobic plate count method specifications of the People's Republic of China.

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