Determination of Tin in Food 1 - National Food Safety Standard
Publish Date: 2015-01-28
Implement Date: 2015-07-28
Issued by: The National Health and Family Planning Commission of the People's Republic of China
Standard Status: currently valid
This is the National Standard of the People's Republic of China, GB5009.16-2014, Determination of Tin in Food, there are two analytical methods. The method 1: hydride atomic fluorescence spectrometry ( AFS ) . The method 2: benzfluorenone colorimetry. This article describes the method 1.
The method 1 of this standard specifies hydride atomic fluorescence spectrometry ( AFS ) for the determination of tin in food. Suitable for determination of tin in food.
The English version of this standard is translated from the Chinese original GB5009.16-2014. In case of discrepancies in the translated version, the original Chinese standard GB5009.16-2014 (Chinese version) shall prevail. This translated version can provide reference for food production enterprises.
Standard Text of GB5009.16-2014
A. Standard Cover of GB5009.16-2014
B. Table of Contents
C. Standard Preface of GB5009.16-2014
Foreword
This standard replaces GB/T 5009.16-2003 "Determination of Tin in Foods".
Compared with GB/T 5009.16-2003, the main changes of this standard are as follows:
—— The name of the standard is changed to "National Food Safety Standard, Determination of Tin in Food";
—— Modified the preparation of standard solutions;
—— Added the sample preparation method for canned food;
—— Modified the description of the instrument measurement part;
—— The quantification limit of method has been increased;
—— Modified the detection limit of the method;
—— Modified the calculation formula.
D. Norma Content of GB5009.16-2014
National Food Safety Standard
Determination of Tin in Food
1. Scope
This standard specifies the determination methods of hydride atomic fluorescence spectrometry and benzfluorenone colorimetry of tin in food. This standard is applicable to the determination of tin in canned solid food, canned beverage, canned jam, canned infant formula and supplementary food.
The method 1: Hydride Atomic Fluorescence Spectrometry
2. Principle
After the sample is digested, the hydride (SNH4) of the tin is generated under the action of sodium boroside. The SNH4 is carried by carrier gas, brought into an atomizer for atomize. Under the irradiation of the tin hollow cathode lamp, the tin atom in the base state is stimulated to high energy state. During the deactivation process, the high-energy tin atom returns to the base state and emits fluorescence with a characteristic wavelength. The fluorescence intensity is proportional to the tin content. It is compared with the solutions of standard series, for quantitative analysis.
3. Reagents and Materials
Note: Unless otherwise specified, the reagents used in this method are all analytical reagent (AR) and the water is the water of grade 2, specified in GB/T 6682.
3.1 Reagents
3.1.1 Sulfuric acid (H2SO4): Guaranteed reagent (Gr).
……
3.2 Reagent Preparation
3.2.1 Mixed solution of nitric acid and perchloric acid (4+1): Measure 400 mL of nitric acid and 100 mL of perchloric acid, and mix well.
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3.3 Standard Chemicals
It is metal tin (Sn) standard with 99.99% purity. It must be a reference material (RM) certified by the state and granted a reference material certificate.
3.4 Preparation of standard solutions
3.4.1 Tin standard solution (1.0mg/L): Accurately weigh 0.1g (accurate to 0.0001g) metal tin standard and place it in a small beaker. ……
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4. Instruments and Equipment
4.1 Atomic fluorescence spectrometer.
4.2 Electric heating plate.
4.3 Electronic balance: the sensing capacity is 0.1mg and 1mg.
5. Analysis Steps
5.1 Sample Preparation
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5.2 Sample Digestion
5.2.1 Weigh 1.0g ~ 5.0g of sample into a conical flask, add 20.0mL of nitric acid + perchloric acid mixed solution (4+1). ……
……
5.3 Instrument reference conditions
Atomic fluorescence spectrometer analysis reference conditions:
—— Negative high voltage: 380V;
—— Lamp current: 70mA;
……
5.4 Preparation of standard series solutions
Standard curve: Pipette 0.00mL, 0.50mL, 2.00mL, 3.00mL, 4.00mL, and 5.00Ml of tin standard solution into a 25mL colorimetric tube respectively. ……
5.5 Instrumental determination
Set the best conditions for the instrument to measure according to 5.3. Set the corresponding parameters according to the model of the instrument used and the workstation, ignite and preheat the instrument. ……
6. Presentation of analysis results
The tin content in the sample is calculated according to formula (1):
---------- (1)
In formula (1):
X —— the tin content in the sample, in milligrams per kilogram (mg/kg);
C1 —— the measured concentration of the sample digestion solution, in nanograms per milliliter (ng/mL);
……
When the calculated result is less than 10mg/kg, keep two digits after the decimal point. When greater than 10mg/kg, …….
7. Precision
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8. Others
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Note: Commonly used Chinese food standards include mandatory national standards (the code starts with "GB") and recommended national standards (the code starts with "GB/T")
If you need other Chinese standards related to food, please "Contact Us". We do our best to find and provide translation services.
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References Articles
1. Principle of Inductively Coupled Plasma-Mass Spectrometer (ICP-MS)
2. Principle of Inductively Coupled Plasma - Optical Emission Spectrometer
3. Determination of Tin in Food 1 - National Food Safety Standard
4. Determination of Tin in Food 2 - National Food Safety Standard
5. Hazard Analysis and Critical Control Point (HACCP) System - General Requirements for Food Processing Plant
6. General Hygienic Regulation for Food Production - National Food Safety Standard
7. GB5749—2022 Standards for Drinking Water Quality
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Related Testing Equipment
1. SUP-70 Inductively Coupled Plasma-Mass Spectrometer ( ICP-MS): can detect dozens of elements and isotopes at one time, ultra-trace analysis, automatic.
2. SUP-70M Vehicle-mounted Inductively Coupled Plasma-Mass Spectrometer: can detect dozens of elements and isotopes in one-time ultra-trace, anti-vibration.
3. SUP-72 Inductively Coupled Plasma-Mass Spectrometer: is a high-sensitivity, rapid food detection and ultra-trace analysis instrument, automatic.
4. SUP-735 Inductively Coupled Plasma-Mass Spectrometer ( ICP-MS /MS, ICP-QQQ ): is a space tandem multi-stage quadrupole mass spectrometer equipped with an inductively coupled plasma.
5. Laboratory Equipment and Instruments: include precision weighing equipment, evaporators, drying ovens and food testing instruments.
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